Programmable control of CRISPR-Cas9 systems by engineering sgRNA as toehold- switchable riboregulators
July 16-20, 2017
Robust control over gene expression is necessary for diverse applications in molecular biology, synthetic biology, and biotechnology. One of the most promising strategies to exert these types of control is the recently developed CRISPR interference (CRISPRi) and activation (CRISPRa) approach, which provides simple and highly effective RNA-based methods for targeted silencing and upregulation of transcription in bacterial and eukaryotic cells. While these current methods are capable of sequence-specific targeting
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Ka-Hei Siu and Wilfred Chen, "Programmable control of CRISPR-Cas9 systems by engineering sgRNA as toehold- switchable riboregulators" in "Biochemical and Molecular Engineering XX", Wilfred Chen, University of Delaware, USA Nicole Borth, Universität für Bodenkultur, Vienna, Austria Stefanos Grammatikos, UCB Pharma, Belgium Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biochem_xx/13