Intracellular secretion analysis of therapeutic antibodies in engineered high- producible CHO cells
July 16-20, 2017
The Chinese Hamster Ovary (CHO) cell is the most commonly used cell line for the production of therapeutic recombinant proteins. The improvements in target gene amplification and culture method have contributed in achieving a very high productivity. Some studies have focused on post-translational secretion processes, and overexpression of proteins which work in the secretion pathway successfully increased the productivity . However, those studies were performed based on the knowledge obtained from the normal, adherent cultured cells, and the detailed secretion processes of recombinant proteins in engineered, suspension cultured cells is still unclear. To clarify problems and to find new targets for a more efficient establishment of high producers, the basic analyses about the secretion in engineered, high-producible CHO cells were performed.
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Kohei Kaneyoshi, Takeshi Omasa, Yuichi Koga, Noriko Yamano, Keiji Uchiyama, and Masayoshi Onitsuka, "Intracellular secretion analysis of therapeutic antibodies in engineered high- producible CHO cells" in "Biochemical and Molecular Engineering XX", Wilfred Chen, University of Delaware, USA Nicole Borth, Universität für Bodenkultur, Vienna, Austria Stefanos Grammatikos, UCB Pharma, Belgium Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biochem_xx/16