Sustainable production of industrially relevant biomonomers: A photosynthetic consortia approach
July 16-20, 2017
Engineering of synthetic microbial consortia has emerged as a new and powerful biotechnology platform. To date, most microbial consortia have focused on biofuel development, though they also have enormous potential in the production of biobased commodity chemicals. This proposal describes a tripartite system in which three microbes of differentiated specializations can convert sunlight, carbon dioxide, and atmospheric nitrogen into chemical precursors for bulk polymer production. This framework offers a novel opportunity for biobased plastic production without energetically or monetarily expensive nutrient inputs, subsequently providing an attractive, sustainable alternative to fossil fuel analogues. Specifically, Azotobacter vinelandii, a nitrogen-fixing bacterium that secretes ammonia, and Synechococcus elongatus, a photosynthetic cyanobacterium that secretes sucrose form a symbiotic chassis hypothesized to support a third producer strain. Escherichia coli and Corynebacterium glutamicum are two archetypal bacterial species previously modified to produce an enormous array of chemicals. Given that both species can naturally grow on ammonia and sucrose, this will facilitate a tailored tripartite system with drop-in target production. These microorganisms have demonstrated production of chemical biopolymers well beyond polyhydroxyalkanoates (PHAs) and polylactic acid (PLA), including varied titers of amino acids, which serve as biomonomers for downstream chemocatalytic bioplastic production. This work bridges a fundamental gap between commercial biofermentation and community engineering, potentially alleviating energetic and economic constraints barring market entry of industrial biopolymer development. The consortium will be studied and optimized for the production of industrially relevant biomonomers as illustrated in Figure 1. Firstly, a computational and experimental investigation will establish a viable tripartite system based on microbial growth.
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David N. Carruthers and Xiaoxia Nina Lin, "Sustainable production of industrially relevant biomonomers: A photosynthetic consortia approach" in "Biochemical and Molecular Engineering XX", Wilfred Chen, University of Delaware, USA Nicole Borth, Universität für Bodenkultur, Vienna, Austria Stefanos Grammatikos, UCB Pharma, Belgium Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biochem_xx/38