Integrated manufacturing with microbial hosts for fast process development and production
September 17-21, 2017
Integrated and continuously operating processes for recombinant protein drugs that rely on non-mammalian hosts offer potential advantages compared to CHO-based platforms for the rapid production of high-quality biopharmaceuticals. This talk will describe insights and technical advances related to the realization of microbial-based production of proteins gained over the past four years in developing a small prototype system for on-demand production of biologics (Figure). Key learnings discussed will include: 1) how deep ‘-omic level’ understanding of a minimal yeast genome can inform both strain engineering and defined media formulation for enhanced production; 2) new platformable approaches for rapid in silico identification of downstream processes that provide highly orthogonal host-related impurity selectivity between individual chromatographic steps; and 3) examples of integration of these elements to enable hands-free production over days with only minor system changes required to accommodate multiple products. In total, holistic design principles and integration of knowledge together facilitate rapid process development and speed to production at scales relevant for a variety of use cases. These examples highlight the opportunities for employing well-defined alternative hosts with tractable genomes in efficient integrated processes for agile manufacturing. Such strategies should complement on-going and existing efforts to convert CHO-based platforms into continuous operations.
Please click Additional Files below to see the full abstract.
J. Christopher Love, "Integrated manufacturing with microbial hosts for fast process development and production" in "Integrated Continuous Biomanufacturing III", Suzanne Farid, University College London, United Kingdom Chetan Goudar, Amgen, USA Paula Alves, IBET, Portugal Veena Warikoo, Axcella Health, Inc., USA Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biomanufact_iii/58