Optical glucose sensor for single use microfluidic reactors
May 7-10, 2017
Glucose measurements are of tremendous interest in biotechnology. Electrochemical and optical methods to measure glucose have been intensively investigated in the last 30 years, in particular for medical diagnostics. A widely used principle is to measure the consumption of oxygen during the oxidation process of glucose facilitated by the enzyme glucose oxidase. The determination of glucose is based on a correlation between the oxygen consumption and the glucose concentration. In our contribution, we demonstrate the integration of a flow-through glucose sensor into microfluidic devices for cell culturing. The sensor consists of three layers: an oxygen sensitive layer, an enzymatic glucose-oxidase layer for glucose induced oxygen consumption and a diffusion layer. The oxygen sensitive layer contains polystyrene particles stained with meso-tetra(4-fluorophenyl)tetrabenzoporphyrin (PtTPTBPF4) embedded in a swellable hydrogel. The second layer contains aggregates of the enzyme glucose oxidase embedded in the same hydrogel. The last and final layer is a plain hydrogel diffusion barrier. By increasing the layer height of the diffusion layer, the dynamic range of the sensor can be tuned.
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Philipp Sulzer, Mayr T, and Altenhuber N, "Optical glucose sensor for single use microfluidic reactors" in "Single-use Technologies II: Bridging Polymer Science to Biotechnology Applications", kta Mahajan (Genentech, Inc., USA) Gary Lye (University College London, UK) Regine Eibl-Schindler (Zurich University of Applied Science, Switzerland) Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biopoly_ii/2