May 7-10, 2017
In the recent years droplet-based microfluidic techniques were developed and used for various screening purposes mainly in the field of life-sciences and biotechnology. Therefore, droplet volumes in a range from pL to μL were used and different technology platforms were necessary to produce the required fluidic devices which made of various materials. Traditionally, lithography combined with etching processes were used to produce precise glass or silicon devices. But, hot embossing and injection molding techniques are better suited for the production of single use devices. Recently, 3D-printing technologies are under development for the generation of individual costumes made microfluidic devices in low numbers for reasonable prices. Here, we present an example for a complex droplet based screening network designed for bacterial screening purposes received by CD-production technologies. General principles of droplet based processing, fluidic devise units and peripheral analytical techniques will be introduced as well as details for the screening of soil bacteria will be given. As an outlook the future microfluidic techniques for monitoring or continuous sampling form large volume bioprocesses will be given.
Alexander Groß, Jialan Cao, Stefen Schneider, J.M. Köhler, Thomas Henkel, Martin Roth, Mark Kielpinsky, and Alexander Mendel, "Single use droplet Based Microfluidics – Screening Tools for Biotechnology and Life-Sciences" in "Single-use Technologies II: Bridging Polymer Science to Biotechnology Applications", kta Mahajan (Genentech, Inc., USA) Gary Lye (University College London, UK) Regine Eibl-Schindler (Zurich University of Applied Science, Switzerland) Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biopoly_ii/36