Microbial cultivation in rocking single-use bioreactors
May 7-10, 2017
The application of single-use bioreactors (SUB) for microbial cultivation, especially of reactor designs beyond the traditional stirred tank, is usually regarded as crucial. Especially the usually low gas mass transfer coefficients are insufficient, however this is not true for 2-dimensionally rocking motion bioreactors like the CELL-tainer®. Volumetric gas mass transfer coefficients (kLa-values) of 600 h-1 are achieved (fig. 1), which allow bacterial fed-batch cultivations up to a cell density of 50 gL-1 at growth rates of 0.3 h-1 w/o any oxygen blending (Junne et al, Chem Eng Technol 2013, 85, p. 57-66). One major asset in this respect are the low maximum shear forces in comparison to stirred tank reactors. This feature might be beneficial when shear sensitive microbes are cultivated, like marine phototrophic and heterotrophic microalgae and filamentous organisms.
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Stefan Junne, Anna-Maria Marbà Ardébol, Peter Neubauer, Vera Meyer, Tutku Kurt, and Howard Ramirez Malule, "Microbial cultivation in rocking single-use bioreactors" in "Single-use Technologies II: Bridging Polymer Science to Biotechnology Applications", kta Mahajan (Genentech, Inc., USA) Gary Lye (University College London, UK) Regine Eibl-Schindler (Zurich University of Applied Science, Switzerland) Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biopoly_ii/38