Hydroperoxide generation in irradiation-sterilized SUS and potential risks of protein oxidation
May 7-10, 2017
Oxidation in polymeric medical implants sterilized by ionizing radiation is by now a well-studied phenomenon. One byproduct of the multitude of oxidative pathways initiated during irradiation of polymers is the generation of hydroperoxide groups bound to polymer chains or fragments (see Figure 1 and reference below). We have discovered that exposure to irradiation-sterilized SUS containers has the potential to induce measurable amounts of oxidation in some protein therapeutics, especially in the context of stability studies having high surface area to volume conditions and/or low protein concentration formulations. This presentation will discuss efforts in our laboratories to quantify the oxidizing species (e.g. hydroperoxides) in SUS containers and explore the parameters that could lead to variability in the amounts present and available to react with product molecules. Using the information gained, we provide a strategy to assess potential risk of protein oxidation in commercial manufacturing operations.
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Matthew Hammond, Samuel Dorey, and Jonathan Cutting, "Hydroperoxide generation in irradiation-sterilized SUS and potential risks of protein oxidation" in "Single-use Technologies II: Bridging Polymer Science to Biotechnology Applications", kta Mahajan (Genentech, Inc., USA) Gary Lye (University College London, UK) Regine Eibl-Schindler (Zurich University of Applied Science, Switzerland) Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biopoly_ii/45