Fluctuations in single-cell organelle size estimates from angular scattering measurements
July 23-26, 2017
Angularly resolved elastic scattering microscopy enables the size distribution of cellular organelles to be estimated non-invasively. By comparing the angular distribution of scattered light to Mie theory models, our group is working towards obtaining quantitative estimates of mean organelle size within single cells. Recent work, for example, established that the lower end of the angular range plays a critical role. Both simulations and measurements demonstrate that lowering the minimum measured angle from 20 degrees to below 15 degrees is essential for obtaining stable size estimates from a single cell.
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Robert L. Draham, Andrew Berger, and Ashley Cannaday, "Fluctuations in single-cell organelle size estimates from angular scattering measurements" in "Advances in Optics for Biotechnology, Medicine and Surgery XV", Peter So, Massachusetts Institute of Technology, USA Kate Bechtel, Triple Ring Technologies, USA Ivo Vellekoop, University of Twente, The Netherlands Michael Choma, Yale University, USA Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biotech_med_xv/16
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