In vivo multiphoton microscopy beyond 1 mm in the brain
July 23-26, 2017
The ability to visualize deep brain structures in vivo with high spatial resolution is of rising interest to investigate neuronal physiology and cerebral vasculature. Optical imaging offers non-invasive, high-resolution in vivo microscopy techniques to observe brain tissue and its surrounding environment. Two-photon fluorescence laser- scanning microscopy (2PM) can overcome depth limitations by using nonlinear excitation. The ideal approach for deep imaging in brain is to use both high energy pulses and longer excitation wavelengths.
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David R. Miller, Ahmed Hassan, Jeremy W. Jarrett, Evan P. Perillo, Boris V. Zemelman, and Andrew K. Dunn, "In vivo multiphoton microscopy beyond 1 mm in the brain" in "Advances in Optics for Biotechnology, Medicine and Surgery XV", Peter So, Massachusetts Institute of Technology, USA Kate Bechtel, Triple Ring Technologies, USA Ivo Vellekoop, University of Twente, The Netherlands Michael Choma, Yale University, USA Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biotech_med_xv/21