Rollerball microendoscope for mosaicking in high-resolution oral imaging
July 23-26, 2017
Only 40% of oral cancers are diagnosed at an early, localized stage, when treatment is most effective . Thus, implementing diagnostic imaging tools for early detection of highgrade dysplasia and cancer may help improve the survival rate of oral cancer patients . The highresolution microendoscope (HRME) is a compact, portable, fiberbased imaging device that can image cell nuclei in tissue labeled with the fluorescent contrast agent proflavine . The HRME allows clinicians to noninvasively image the size, shape and distribution of epithelial cell nuclei in vivo, enabling realtime evaluation of potentially neoplastic lesions . The primary limitation of the HRME is the small field of view of its fiber probe (720 μm), which makes it timeconsuming to examine large areas of tissue. Mosaicking algorithms have previously been implemented to allow realtime generation of image mosaics during HRME imaging, thus interrogating a larger field of view than the fiber probe’s diameter . However, this approach has had limited success in vivo due to the practical difficulty of translating the fiber probe across the tissue in a smooth, controlled manner in order for the mosaicking software to function properly. Here we report the construction and initial testing of a rollerball HRME probe that permits smooth, rolling translation across the tissue surface while maintaining image quality with subcellular resolution. The rollerball HRME consists of a standard HRME probe interfaced with a rollerball mechanism. The mechanism is composed of two 5mm sapphire ball lenses enclosed within a 3D printed penlike casing. The ball lenses serve as an optical relay, while the distal ball lens also serves as a rolling contact point with the tissue surface. Figure 1 shows the use of the rollerball HRME to generate a realtime mosaic of a calibration target (field finder slide) as it rolls across the surface of the target. Figure 2 shows the use of the rollerball HRME to generate a realtime mosaic showing cell nuclei on the lateral tongue of a healthy volunteer as it rolls across the tissue surface. The rollerball HRME will allow clinicians to more rapidly examine large areas of tissue with subcellular resolution, potentially aiding in the early detection of highgrade oral dysplasia and cance.
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Nicole Sevilla, Rebecca Richards-Kortum, Richard . A. Schwarz, Timothy Quang, Katelin D. Cherry, Nadarajah Vigneswaran, and Ann M. Gillenwater, "Rollerball microendoscope for mosaicking in high-resolution oral imaging" in "Advances in Optics for Biotechnology, Medicine and Surgery XV", Peter So, Massachusetts Institute of Technology, USA Kate Bechtel, Triple Ring Technologies, USA Ivo Vellekoop, University of Twente, The Netherlands Michael Choma, Yale University, USA Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/biotech_med_xv/24