A reference genome for the Chinese hamster based on a hybrid assembly strategy
May 6-11, 2018
Accurate and complete genome sequences are essential in biotechnology, especially to facilitate genome-based cell engineering efforts for the main production host of biotherapeutic proteins, Chinese hamster ovary (CHO) cells. While genome-enabled CHO cell engineering efforts promise to enhance drug production, the current genome assemblies for Cricetulus griseus, the Chinese hamster, are highly fragmented and replete with gap sequences and misassemblies, consistent with most short-read based assemblies. Here we have completely re-sequenced the Chinese hamster, C. griseus, using Single Molecule Real Time (SMRT) long-read technology and merged this assembly with Illumina-based assemblies.
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Kelvin H. Lee, Oliver Rupp, Sven Griep, Karina Brinkrolf, Alexander Goesmann, Kelley Heffner, Haiping Hou, Michael Betenbaugh, Shangzhong Li, Mojtaba Samoudi, Nathan Lewis, Heena Dhiman, Inmaculada Hernandez, Vaibhav Jadhav, Nicole Borth, Madolyn L. MacDonald, Shawn Polson, and Brewster Kingham, "A reference genome for the Chinese hamster based on a hybrid assembly strategy" in "Cell Culture Engineering XVI", A. Robinson, PhD, Tulane University R. Venkat, PhD, MedImmune E. Schaefer, ScD, J&J Janssen Eds, ECI Symposium Series, (2018). http://dc.engconfintl.org/ccexvi/1