Newly established cell lines derived from Chinese hamster for production of biologics
May 6-11, 2018
The Chinese hamster (Cricetulus griseus) is a species of hamster that was used as a laboratory animal more than 50 years ago. The Chinese hamster ovary (CHO) cell line was established in 1957 by Puck et al. and maintained in ex vivo conditions (1). CHO cells are now a workhorse for recombinant biopharmaceutical production. Puck et al. continuously cultivated lung, kidney, spleen and ovary-derived cells for more than 10 months using serum medium. In this study, we established a cell line from female Chinese hamster tissues: lung, kidney and ovary. Primary cells were obtained from these tissues and maintained for several months or more (Figure 1). We were ultimately able to construct three immortal cell lines, CHL-YN (fibroblast) from lung, CHK-Q (epitheliocyte) from kidney and CHO-MK (epitheliocyte) from ovary, respectively. Infinite proliferation of these cell lines is obtained by spontaneous transformation.
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Takeshi Omasa and Noriko Yamano, "Newly established cell lines derived from Chinese hamster for production of biologics" in "Cell Culture Engineering XVI", A. Robinson, PhD, Tulane University R. Venkat, PhD, MedImmune E. Schaefer, ScD, J&J Janssen Eds, ECI Symposium Series, (2018). http://dc.engconfintl.org/ccexvi/177