Evolution of TFF-based perfusion: A path towards non product sieving and direct chromatography integration
May 6-11, 2018
Manufacturers of therapeutic proteins are becoming increasingly interested in continuous bioprocessing. Via uninterrupted medium exchange, perfusion cell culture yields higher volumetric productivity than traditional batch/fed-batch processes with 2 to 5 fold higher cell density and cultivation duration. In addition, an uninterrupted removal of therapeutic proteins promotes a desired alignment with integrated continuous purification processes to eliminate large hold tanks. Hollow fiber tangential flow filtration (TFF) is commonly used for cell retention in perfusion cell culture. In these perfusion systems the major limitation can be inefficient or decaying product sieving. This increases product’s retention time in the bioreactor, which may detrimentally affect the target protein quality attributes and decrease yield.
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Nuno D. S. Pinto, "Evolution of TFF-based perfusion: A path towards non product sieving and direct chromatography integration" in "Cell Culture Engineering XVI", A. Robinson, PhD, Tulane University R. Venkat, PhD, MedImmune E. Schaefer, ScD, J&J Janssen Eds, ECI Symposium Series, (2018). http://dc.engconfintl.org/ccexvi/235