Large scale production and characterization of exosomes
May 6-11, 2018
Exosome-based therapeutics are rapidly evolving as a new modality with a promising potential in multiple clinical areas. Successful implementation requires development of robust large-scale processes for the manufacture of highly purified material. However, the complexity and heterogeneity of exosomes pose significant R&D challenges. Here, we present the successful development of a manufacturing process using immortalized human cells, currently operating at commercial scale. Contrary to traditional ultracentrifugation approaches for exosome production, our process is based on modern biochemical engineering principles, resulting in a robust and scalable operation. The related analytics and characterization methods are also discussed.
Konstantin Konstantinov, "Large scale production and characterization of exosomes" in "Cell Culture Engineering XVI", A. Robinson, PhD, Tulane University R. Venkat, PhD, MedImmune E. Schaefer, ScD, J&J Janssen Eds, ECI Symposium Series, (2018). http://dc.engconfintl.org/ccexvi/238