High shear stress from a resonance phenomenon in Wave bioreactor revealed by computational fluid dynamics simulation
January 27-31, 2019
Wave bioreactors are getting a wide acceptance for the culture of human cells. These bioreactors are attractive for sensitive cells such as stem cells or immune cells, in suspension, aggregates or adherently growing on microcarriers. The optimization of the mixing, the oxygen transfer rate and the shear stress require a deep understanding of the hydrodynamics taking place in the Wave bioreactor bag. In the present study, we perform numerical simulations (Ansys-FLUENT) to characterize the flow conditions in a 10L cellbag . The numerical simulations were carried out to investigate the fluid structures for nine different operating conditions of rocking speed and angle. The mixing and the shear stress induced by the liquid motion were studied. We observed that these increased with the cellbag angle from 4˚ to 7˚ but that the highest rocking velocities were not systematically associated with the highest mixing and shear stress. As a matter of fact, the lowest studied rocking speed, 15 rpm, generated the highest fluid velocity, mixing and shear stress compared to the higher speeds of 22 and 30 rpm. It was concluded that a resonance phenomenon was responsible for this behavior.
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Veronique Chotteau, Caijuan Zhan, Erika Hagrot, and Luca Brandt, "High shear stress from a resonance phenomenon in Wave bioreactor revealed by computational fluid dynamics simulation" in "Advancing Manufacture of Cell and Gene Therapies VI", Dolores Baksh, GE Healthcare, USA Rod Rietze, Novartis, USA Ivan Wall, Aston University, United Kingdom Eds, ECI Symposium Series, (2019). http://dc.engconfintl.org/cell_gene_therapies_vi/12