Characterization of CAR-T transduction parameters using a lentiviral vector
January 27-31, 2019
Production of chimeric antigen receptor T cells (CAR-T) can be accomplished using a viral vector to deliver the transgene. Lentiviral vectors (LVV) are commonly used for transduction due to their safety profile, stable transduction, ease of use, and ability to transduce both dividing and nondividing cells. Characterization studies were conducted to identify parameters within the manufacturing process that could impact transduction efficiency, such as the multiplicity of infection (MOI), application time after T cell activation, and LVV degradation profile. Optimizing these parameters will result in improved reproducibility within the manufacturing process and the efficient use of LVV material. Response curves for transduction efficiency, vector copy number and potency exhibited similar patterns to one another; signals increased with increasing MOI until reaching a plateau.
Stefanie Shahan, Sherry Zhou, Ryan Shorr, and Thomas Brieva, "Characterization of CAR-T transduction parameters using a lentiviral vector" in "Advancing Manufacture of Cell and Gene Therapies VI", Dolores Baksh, GE Healthcare, USA Rod Rietze, Novartis, USA Ivan Wall, Aston University, United Kingdom Eds, ECI Symposium Series, (2019). http://dc.engconfintl.org/cell_gene_therapies_vi/31