Dissolvable microcarriers for efficient cell production and recovery
January 15-19, 2017
Cell scale-up on microcarriers is an increasingly important technique for bioprocess applications. However, the harvest, separation, and concentration of final product (e.g. cell, virus, or antibody) can be time and resource consuming. To address these issues, Corning has developed a new generation of microcarriers that can be quickly dissolved, allowing easy collection of grown cells or secreted products without the need for microcarrier separation. Here we present potential applications for dissolvable microcarriers. Human mesenchymal stem cells (hMSCs), MRC5, Vero, and customer-specific cell types were grown in spinner flask cultures or bioreactors on dissolvable microcarriers. For all cell types evaluated, growth performance on dissolvable microcarriers was equivalent to other commercially available microcarriers, and high quality cells were recovered without the need for microcarrier separation.
Todd Sciortino, Jennifer Weber, Jeffrey Scibek, and Martial Hervy, "Dissolvable microcarriers for efficient cell production and recovery" in "Scale-up and Manufacturing of Cell-based Therapies V", Tom Brieva, Celgene Cellular Therapeutics William Miller, Northwestern University Chris Mason, University College London Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/cellbasedtherapies_v/13