Rapid human T cell expansion using gas-permeable bags in the Eppendorf New BrunswickTM S41i CO2 incubator shaker
January 15-19, 2017
Recent advances in cellular therapy have greatly increased the demand for T cell expansion techniques. T cells must be expanded rapidly to achieve high cell densities while maintaining high viability and T cell identity. One of the best cell culture tools for large-scale expansion is stirred-tank bioreactors. However, cell culture in bioreactors requires a relatively large number of cells for inoculation. We developed a -method using gas-permeable bags to produce a sufficient number of T cells for the inoculation of bench-scale bioreactors.
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Ma Sha, Amanda Suttle, and Stacey Willard, "Rapid human T cell expansion using gas-permeable bags in the Eppendorf New BrunswickTM S41i CO2 incubator shaker" in "Scale-up and Manufacturing of Cell-based Therapies V", Tom Brieva, Celgene Cellular Therapeutics William Miller, Northwestern University Chris Mason, University College London Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/cellbasedtherapies_v/41