Title
Preferentially selecting cellular metabolism and improving productivity by controlling do and Pco2
Conference Dates
May 8-13, 2016
Abstract
Cells utilize glucose as their main resource for deriving energy through ATP production. The quantity of ATP generated depends on the metabolic pathways that are employed, aerobic glucose metabolism or anaerobic glucose metabolism. Using our bench top bioreactor model, we have shown these two metabolic pathways can be preferentially selected by controlling the desired cell culture DO and pCO2, and productivity was increased as a result. The DO and pCO2 controlling strategy was implemented in at-scale bioreactors and yielded the expected metabolic and productivity outcome
Recommended Citation
Jiayi Zhang, Ryan Cassidy, Mark Emanuele, Gonzalo Milet, and Lada Laenen, "Preferentially selecting cellular metabolism and improving productivity by controlling do and Pco2" in "Cell Culture Engineering XV", Robert Kiss, Genentech Sarah Harcum, Clemson University Jeff Chalmers, Ohio State University Eds, ECI Symposium Series, (2016). https://dc.engconfintl.org/cellculture_xv/148