Protein engineering of Candida rugosa lipase
September 24-28, 2017
Lipases (EC 18.104.22.168) catalyze the hydrolysis of emulsified long-chain triacylglycerol at the lipid–water interface. Candida rugosa lipase (CRL) is one of important industrial enzymes that are widely used in biotechnological applications such as the production of fatty acids and the synthesis of various esters. The catalytic efficiency and substrate specificity are seemed to be the key factors for industrial applications. Several approaches have been used to increase the stability of different lipases. For example, the immobilized CRL on carriers led to significant improvement in the catalyst’s activity and stability (Ref 1). In this study, we have focused on protein engineering to improve the properties of CRL.
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Satoru Ishihara, Tetsuya Takahashi, Satoshi Koikeda, Kazunori Yoshida, and Kazuhiko Ishikawa, "Protein engineering of Candida rugosa lipase" in "Enzyme Engineering XXIV", Pierre Monsan, Toulouse White Biotechnology, France Magali Remaud-Simeon, LISBP-INSA, University of Toulouse, France Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/enzyme_xxiv/4