Protein engineering of Candida rugosa lipase

Conference Dates

September 24-28, 2017


Lipases (EC catalyze the hydrolysis of emulsified long-chain triacylglycerol at the lipid–water interface. Candida rugosa lipase (CRL) is one of important industrial enzymes that are widely used in biotechnological applications such as the production of fatty acids and the synthesis of various esters. The catalytic efficiency and substrate specificity are seemed to be the key factors for industrial applications. Several approaches have been used to increase the stability of different lipases. For example, the immobilized CRL on carriers led to significant improvement in the catalyst’s activity and stability (Ref 1). In this study, we have focused on protein engineering to improve the properties of CRL.

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