Rieske non-heme dioxygenases: Versatile biocatalysts for the generation of vicinal Cis- Diols
September 24-28, 2017
Vicinal cis-diols are a common motif in many biologically active natural products and important intermediates in the flavor and fragrance as well as pharmaceutical industries. Most of the chemical approaches for cis-dihydroxylations are based on transition-metal catalysts like osmium and manganese, which are expensive and toxic. Hence, the enzyme-catalyzed asymmetric dihydroxylation is a powerful tool to overcome the obstacles encountered using such chemical approaches. With the Rieske non-heme dioxygenases (ROs) a promising biocatalytic alternative to generate very selectively vicinal cis-diols is at our disposal. Also known as the non-heme analog to P450 monooxygenases1, these enzymes are multicomponent systems, which only need molecular oxygen for the vicinal cis-dihydroxylation (s. Figure 1).
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Julia M. Halder, Bernhard Hauer, and Bettina M. Nestl, "Rieske non-heme dioxygenases: Versatile biocatalysts for the generation of vicinal Cis- Diols" in "Enzyme Engineering XXIV", Pierre Monsan, Toulouse White Biotechnology, France Magali Remaud-Simeon, LISBP-INSA, University of Toulouse, France Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/enzyme_xxiv/64