Engineering a robust cyclohexanone monooxygenase for the production of methyl propanoate
September 24-28, 2017
Cyclohexanone monooxygenase (EC 126.96.36.199) from Acinetobacter calcoaceticus (AcCHMO) catalyzes the Baeyer-Villiger oxidation of 2-butanone, producing both ethyl acetate and methyl propanoate (Fig. 1A). Methyl propanoate is of industrial interest as a precursor of acrylic plastic. We have replaced various residues near the substrate and NADP+ binding sites in AcCHMO using saturation mutagenesis with the aim of increasing both the activity on 2-butanone and the methyl propanoate/total product ratio. Whole cell biotransformations were prepared for the resulting libraries and the analyses were carried out by headspace GC analysis. A higher conversion yield (92%) and kcat value (0.5 s-1) than wild type AcCHMO (52% and 0.3 s-1, respectively) were observed for T56S AcCHMO. I491A AcCHMO exhibited a significant improvement over the wild type enzyme in the desired regioselectivity using 2-butanone as a substrate (40% vs. 26% methyl propanoate, respectively). The T56S/I491A double mutant combined the beneficial effects of both mutations (Fig. 1B).
Recently, we reported on the discovery, characterization, and crystal structure determination of a CHMO from Thermocrispum municipale (TmCHMO). A Ser residue was found in TmCHMO at the equivalent position to that of AcCHMO T56. The TmCHMO I493, equivalent to AcCHMO I491, was replaced with an Ala by site-directed mutagenesis. The resulting mutant exhibited a similar activity and regioselectivity to those observed for T56S/I491A AcCHMO using the substrate 2-butanone. This study shows that even for a relatively small aliphatic substrate, regioselectivity can be tuned by structure-inspired enzyme engineering in two different CHMOs. Beneficial mutations previously carried out for AcCHMO, or other CHMOs, may be repeated in TmCHMO achieving similar effects. This is very attractive for biocatalysis since TmCHMO is significantly more thermostable and solvent tolerant than all CHMOs described so far.
.Please click Additional Files below to see the full abstract.
Elvira Romero, Hugo L. van Beek, Marco W. Fraaije, J. Rubén Gómez Castellanos, and Andrea Mattevi, "Engineering a robust cyclohexanone monooxygenase for the production of methyl propanoate" in "Enzyme Engineering XXIV", Pierre Monsan, Toulouse White Biotechnology, France Magali Remaud-Simeon, LISBP-INSA, University of Toulouse, France Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/enzyme_xxiv/94