Engineering of Corynebacterium glutamicum for the secretory production of recombinant proteins via Tat-dependent pathway
March 4-8, 2018
Corynebacterium glutamicum, which has been an industrial producer of various L-amino acids, nucleic acids, and vitamins, is now also regarded as a potential host for the secretory production of recombinant proteins since it exhibits numerous ideal features for protein secretion: (i) it has a single cellular membrane as a gram-positive bacterium, which allows proteins to be easily secreted into the extracellular medium. (ii) C. glutamicum secretes only a few endogenous proteins into the culture medium, which allows the simpler purification of target proteins in downstream process. (iii), secreted proteins from C. glutamicum can be kept stable because extracellular protease activity is rarely detectable. To harness its potential as an industrial platform for recombinant protein production, the development of an efficient secretion system is necessary. To achieve this goal first, we engineered several genetic parts in C. glutamicum: (i) synthetic promoters, (ii) plasmid copy number, (iii) signal peptides, (iv) co-expression of secretion machinery proteins. Using the engineered host-vector systems, gram-scale production of recombinant proteins could be achieved in fed-batch cultivation.
Jae Woong Choi, Sung Sun Yim, and Ki Jun Jeong, "Engineering of Corynebacterium glutamicum for the secretory production of recombinant proteins via Tat-dependent pathway" in "Microbial Engineering", Prof. Eli Keshavarz-Moore , University College London, England Dr. Barry Buckland, BiologicB, USA Eds, ECI Symposium Series, (2018). http://dc.engconfintl.org/microbial/18