Systems metabolic engineering of Corynebacterium glutamicum and Bacillus methanolicus for production of new products from alternative carbon sources
March 4-8, 2018
Amino acid production amounts to about 2 million tons of L-lysine and 3 million tons of L-glutamate per year . Corynebacterium glutamicum is widely used in industry for amino acid production from sugars, while the methylotrophic Bacillus methanolicus produces L-lysine and L-glutamate from methanol with titers of about 50 g/L . Both microbial hosts have been developed for production of specialty amino acids and amines.
Specifically, I will present new tools for metabolic engineering of B. methanolicus and C. glutamicum including CRISPRi/dCas9, sigma factor engineering, genome reduction, induction withphotocaged IPTG, theta-type replicating vectors and biosensors [3-10]. On the other hand, I will describe examples of metabolic engineering of these hosts for the production of specialty amino acids and diamines. C. glutamicum strains for the production of L-pipecolic acid have been constructed  and these were improved a titer of 14.4 g L-1, a volumetric productivity of 0.21 g L-1 h-1 and an overall yield of 0.20 g g-1 . Moreover, access to production of L-pipecolic acid from glucose, glycerol, xylose, glucosamine, and starch has been enabled. Methanol-based production of the non-proteinogenic amino acid -amino butyric acid (GABA), that finds application also as precursor for bioplastics, has been achieved with B. methanolicus . Efficient production of GABA from hexose and pentose sugars has been realized and optimized in recombinant C. glutamicum strains as well [14,15]. Both for C. glutamicum and B. methanolicus characterization and engineering of product export have been instrumental [16,17].
In conclusion, the microbial cell factories B. methanolicus and C. glutamicum have been programmed for efficient and sustainable production of specialty amino acids and amines from diverse carbon feedstocks to foster their biotechnological applications.
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Volker F. Wendisch, "Systems metabolic engineering of Corynebacterium glutamicum and Bacillus methanolicus for production of new products from alternative carbon sources" in "Microbial Engineering", Prof. Eli Keshavarz-Moore , University College London, England Dr. Barry Buckland, BiologicB, USA Eds, ECI Symposium Series, (2018). http://dc.engconfintl.org/microbial/61