Integrating Nanomembrane Separation with Plasmonic Detection for Real-Time Cell Culture Monitoring
March 5-10, 2017
To further understand cellular responses to drug treatment the dynamics of a reduced secretome shall be investigated. Currently there is no method for the detection of secreted small molecules in real time, label-free and with a high resolution. We present a novel design, which integrates nanopore filtration technology with highly sensitive plasmonic detection that allows real time monitoring of filtered molecules with a high spatial resolution and label free. The cell culture chamber is separated from the site of detection only by our biocompatible nanomembrane filter with a thickness of less than 100 nm to exclude the majority of background signals from the cell culture. The fast filtration of the cell culture constituents through the nanomembrane to the detector allows the observation of the dynamics of secreted molecules during cell culture and/or drug application. The setup offers new possibilities for drug screening and cell assays and may reveal new insights into cell signaling and drug responses. This setup shall be used to monitor cell culture or tissue culture without the necessity of labeling. This can be particularly important for the very popular “organ-on-a-chip” or “patient-on-a-chip” approaches to monitor tissue reactions to drug treatments with a high spatial resolution.
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Stephan Hinterberger, Agnes Rodler, Rupert Tscheliessnig, Luke P. Lee, and Alois Jungbauer, "Integrating Nanomembrane Separation with Plasmonic Detection for Real-Time Cell Culture Monitoring" in "Separations Technology IX: New Frontiers in Media, Techniques, and Technologies", Kamalesh K. Sirkar, New Jersey Institute of Technology, USA Steven M. Crame, Rensselaer Polytechnic Institute, USA João G. Crespo, LAQV-Requimte, FCT-Universidade Nova de Lisboa, Caparica, Portugal Marco Mazzotti, ETH Zurich, Switzerland Eds, ECI Symposium Series, (2017). http://dc.engconfintl.org/separations_technology_ix/15