September 23-26, 2018
Mixers are employed throughout all parts of bioprocessing. Single-use mixers (S.U.M.) are commonly used for upstream operations including media formulation and hydration, media holding, sterile filtration and downstream operations including media and product storage, viral inactivation, buffer preparation, and resin/slurry preparation for column packing. This study presents the automation of both simple and complex mixing procedures using the Thermo Fisher Touchscreen Control Panel. Also investigated are the selection method for scalable mixing parameters allowing normalized mixing performance across all single use mixer sizes. The following applications are demonstrated in this study:
- Characterization of scalable mixing parameters by comparing power input per volume and T95 blend time criteria
- Automation of a basic mixing process control variables including temperature, agitation , pump rate, flow valves, line pressure, and filtration effeciency using the Touchscreen Electrical Panel
- Automation of a complex viral inactivation process including multiple pH and temperature shifts
This work demonstrates best practices for mixing in bioprocessing unit operations including use of the Touchscreen Control Panel for automation thus minimizing operator intervention and process variance while maximizing the quality and traceability of data gathered during the mixing process thus improving overall lab efficiency.
Jordan Cobia, Kevin Mullen, and Becky Staerk, "Mixing Operations for 50 L to 2000 L Single-Use Mixer: Liquid-Liquid Mixing Characterization and Slurry Suspension" in "Single-Use Technologies III: Scientific and Technological Advancements", Weibing Ding, Amgen Martina Micheletti, University College London Robert Repetto, Pfizer Eds, ECI Symposium Series, (2018). http://dc.engconfintl.org/sut_iii/29