Custom open polyethersulfone ultrafiltration membranes for vaccines
June 12-17, 2016
Vaccines save millions of lives every year, and improve the quality of life for countless others. Ultrafiltration (UF) is a size-based separation that is used in nearly all vaccine processes for product concentration, impurity clearance, and buffer adjustment. Polyethersulfone (PES) UF membranes, such as Biomax® from MilliporeSigma, are widely used in the industry. An advancement in the PES membrane manufacturing process can enable better uniformity, improved control of the membrane pore size, and the capability to produce custom variants that could enhance yield, purity, efficiency, and economics in new or existing vaccine processes.
The poster highlights the new capability by showing the relative retention of various pneumococcal polysaccharides across a range of membrane pore sizes or nominal molecular weight cutoffs. A custom membrane would allow the vaccine manufacturer to maximize the yield while maintaining acceptable purity. In conjugated polysaccharide (CPS) vaccines like those for pneumonia and meningitis, bacterial polysaccharides (PS) are chemically linked or conjugated to carrier proteins (CP), e.g. diphtheria or tetanus toxoid to enhance the immune response beyond that of the PS alone. UF membranes are used for the concentration and purification of each component, and are critical for the supply of vaccines worldwide.
Please click Additional Files below to see the full abstract.
Clifton Ngan, "Custom open polyethersulfone ultrafiltration membranes for vaccines" in "Vaccine Technology VI", Laura Palomares, UNAM, Mexico Manon Cox, Protein Sciences Corporation, USA Tarit Mukhopadhyay, University College London, UK Nathalie Garçon, BIOASTER Technology Research Institute, FR Eds, ECI Symposium Series, (2016). http://dc.engconfintl.org/vaccine_vi/85