Safe and green, the hyperbar inactivation
June 12-17, 2016
To make a safe, effective vaccine, the process must be able to completely inactivate the pathogenic microorganism while retaining the organism’s immunogenic potential. The classic method, using toxic chemicals such as thiomersal or formaldehyde, has many disadvantages: the safety constraints needed to handle these products, the potential for a residual presence in the final product and a possible change in the antigen structure impacting the immune response. A new technology assessed in collaboration with Merial and TOP Industry makes it possible to design a patented new type of equipment dedicated for the inactivation of any kind of bacteria (Bordetella pertussis, Erysipelothrix rhusiopatiae, Escherichia coli, Salmonella…), while maintaining the bacterial cell structure. This new process consists in applying high hydrostatic pressure to the pathogenic microorganism. The inactivation of the microorganism is completed after several minutes under high pressure, whereas the classical method can require several days. This new technology dramatically reduces the duration of this critical step, improves the safety of the patient by avoiding the use of toxic chemicals and potentially reduces the dose amount needed for the vaccination while improving the quality of the antigens. The poster will present the technology, some of the trials performed to inactivate these bacterias and the results obtained.
Fabien Lux, "Safe and green, the hyperbar inactivation" in "Vaccine Technology VI", Laura Palomares, UNAM, Mexico Manon Cox, Protein Sciences Corporation, USA Tarit Mukhopadhyay, University College London, UK Nathalie Garçon, BIOASTER Technology Research Institute, FR Eds, ECI Symposium Series, (2016). http://dc.engconfintl.org/vaccine_vi/90