Slow growth rate triggered transition to a pseudohyphal lifestyle of the protein production host Pichia pastoris
July 14-18, 2019
Specific growth rate is an important process control parameter for industrial protein production. In the widely used yeast protein production host Pichia pastoris, growth rate is known to significantly impact protein expression and secretion . In that regard, glucose-limited chemostat cultivations carried out over a wide range of specific growth rates have revealed that slow growth rates can trigger a pseudohyphal phenotype in P. pastoris . Such phenotypes are undesirable during large-scale protein production processes since they can lead to foam production. In Saccharomyces cerevisiae pseudohyphal growth is controlled by FLO11, a member of the FLO gene family, which is a group of genes encoding cell surface proteins responsible for conferring a diverse array of adhesion-related phenotypes and reported to be controlled by epigenetic mechanisms. P. pastoris also carries a number of FLO genes but their functions and regulatory patterns are yet unknown. Thus, we set out to investigate this gene family to shed some light on how pseudohyphal growth and other adhesion phenotypes are triggered and regulated in P. pastoris.
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Sonakshi De, Markus Buchetics, Corinna Rebnegger, Diethard Mattanovich, and Brigitte Gasser, "Slow growth rate triggered transition to a pseudohyphal lifestyle of the protein production host Pichia pastoris" in "Biochemical and Molecular Engineering XXI", Christina Chan, Michigan State University, USA Mattheos Koffas, RPI, USA Steffen Schaffer, Evonik Industries, Germany Rashmi Kshirsagar, Biogen, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/biochem_xxi/25