Continuous aqueous two-phase system extraction using oscillatory flow reactor
October 6-10, 2019
Biotechnology has allowed the development of completely new products from health to food industry. The quality of the process since cell culture until formulation is crucial in their application. Nowadays, almost all biological products are still produced entirely in batch mode, which brings some drawbacks that can be overcome, operating in continuous mode. The continuous downstream processing can bring many advantages such as: automation and integration of unit operations, lower operator interaction with the equipment and consequently lower chance of errors; possibility of recycling buffers and auxiliary material; improvement of product quality, due to the greater uniformity of the process time; and increase of the process productivity.
The usage of aqueous two-phase systems (ATPS) have been proven as an efficient operation for the clarification and purification of biological products, but despite this fact ATPS have had a limited use at large scale. On the one hand, ATPS extraction uses aqueous solutions that provide an ideal environment for biological molecules, on the other, the high salt concentrations and the cost of some polymers are the main drawbacks that make this process less cost-competitive comparing with others; however, the majority of ATPS extractions are still done in batchwise mode.
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Diogo Faria, Ana Azevedo, Raquel Aires Barros, and António Ferreira, "Continuous aqueous two-phase system extraction using oscillatory flow reactor" in "Integrated Continuous Biomanufacturing IV", Veena Warikoo, Roche, USA Alois Jungbauer, BOKU, Austria Jon Coffman, Boehringer Ingelheim, USA Jason Walther, Sanofi, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/biomanufact_iv/16