Continuous mode of production for two classes of defective interfering influenza A virus particles as antiviral candidates
October 6-10, 2019
Influenza A virus (IAV) is a major human pathogen with a high mutation rate that causes annual epidemics. Defective interfering particles (DIPs) are naturally occurring IAV mutants that are responsible for low influenza virus yields in continuous passaging. Due to that, previous research suggested that DIPs may be utilized as an antiviral agent . In contrast to infectious influenza standard virus (STV), DIPs typically contain a large internal deletion in at least one of the eight genomic viral RNA (vRNA) segments. For such a DIP, named DI244, protection of ferrets against pandemic influenza A virus was shown . Furthermore, we have recently reported on a novel type of IAV-derived DIP, called OP7 virus, which only contains nucleotide substitutions in segment 7 vRNA instead of large internal deletions . Hence, the focus of this work was to evaluate cell-based production in continuous mode for both DI244 and the newly discovered OP7 DIP.
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Marc Hein, Felipe Tapia, Yvonne Genzel, Sascha Young Kupke, and Udo Reichl, "Continuous mode of production for two classes of defective interfering influenza A virus particles as antiviral candidates" in "Integrated Continuous Biomanufacturing IV", Veena Warikoo, Roche, USA Alois Jungbauer, BOKU, Austria Jon Coffman, Boehringer Ingelheim, USA Jason Walther, Sanofi, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/biomanufact_iv/76