Integrated optical sensors for disposable microfluidics
May 7-10, 2017
Optical chemical sensors are established process monitoring tools in industry and research laboratories. Optical chemical sensors basically comprise of luminescent indicator dye based in a host polymer. They are easy to integrate, non-invasive, do not need any reference element and can be read-out contactless from outside. However, to fully exploit the potential in microfluidic or organ-on- chip devices, the sensors have to fulfil several demands including high brightness, capability to be applied as thin film, excellent photo-stability, cheap and accurate read-out systems, ease in use (simple calibration and drift free), simple mass production compatible preparation steps, compatibility with the chip materials, resistance towards γ-sterilisation and no toxicity. We present sensors for oxygen and pH fulfilling these demands. Our sensors can be excited with red-light and emit light in the near infra-red range (<700 nm). This suppresses background fluorescence and scattering from biological material. Sensor layers or spots are deposited with inkjet-based micro-dispensing or air-brush spraying with good adherence on glass or polymeric materials. A modified miniaturized phase-fluorimeter in a foot-print of a memory stick enables the read-out of sensor sizes below 100 micrometers. The sensor enable dynamic cell culturing and monitoring of cell metabolism in a microfluidic environment. We will give examples of oxygen sensors in a organ-on-chip model and pH sensors in cell cultures.
Please click Additional Files below to see the full abstract.
Torsten Mayr, Martin Strobl, Martin Strobl, Martin Strobl, Martin Strobl, Birgit Ungerböck, and Birgit Ungerböck, "Integrated optical sensors for disposable microfluidics" in "Single-use Technologies II: Bridging Polymer Science to Biotechnology Applications", kta Mahajan (Genentech, Inc., USA) Gary Lye (University College London, UK) Regine Eibl-Schindler (Zurich University of Applied Science, Switzerland) Eds, ECI Symposium Series, (2017). https://dc.engconfintl.org/biopoly_ii/50