Optimizing field-of-view of deep-tissue scanning microscopy
July 23-26, 2017
For centuries, the optical microscope has been a crucial instrument for new biological findings, as microscopes were the first devices allowing to observe the internal processes of the cell. Unfortunately, this observation requires the use of thin samples, as biological tissue scatters the incoming light, resulting in a blurred image. An ever increasing number of deep-tissue imaging technique have pushed the penetration depth of the optical microscope. Methods such as adaptive optics  allow focusing inside biological tissue by correcting for scattering introduced by the sample. However, adaptive optics methods can only correct for image distortions caused by scattering over a single small area (i.e., field-of-view) within tissue.
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Gerwin Osnabrugge, Roarke Horstmeyer, Ioannis N. Papadopoulos, and Ivo M. Vellekoop, "Optimizing field-of-view of deep-tissue scanning microscopy" in "Advances in Optics for Biotechnology, Medicine and Surgery XV", Peter So, Massachusetts Institute of Technology, USA Kate Bechtel, Triple Ring Technologies, USA Ivo Vellekoop, University of Twente, The Netherlands Michael Choma, Yale University, USA Eds, ECI Symposium Series, (2017). https://dc.engconfintl.org/biotech_med_xv/11