Fluctuations in single-cell organelle size estimates from angular scattering measurements

Conference Dates

July 23-26, 2017


Angularly resolved elastic scattering microscopy enables the size distribution of cellular organelles to be estimated non-invasively. By comparing the angular distribution of scattered light to Mie theory models, our group is working towards obtaining quantitative estimates of mean organelle size within single cells. Recent work, for example, established that the lower end of the angular range plays a critical role. Both simulations and measurements demonstrate that lowering the minimum measured angle from 20 degrees to below 15 degrees is essential for obtaining stable size estimates from a single cell.

Please click Additional Files below to see the full abstract.

25.pdf (222 kB)

This document is currently not available here.