ECI Digital Archives

Title

In vivo multiphoton microscopy beyond 1 mm in the brain

Authors

David R. Miller, The University of Texas at Austin, USAFollow
Ahmed Hassan, The University of Texas at Austin, Biomedical Engineering
Jeremy W. Jarrett, The University of Texas at Austin, Biomedical Engineering
Evan P. Perillo, The University of Texas at Austin, Biomedical Engineering
Boris V. Zemelman, The University of Texas at Austin, Biomedical Engineering
Andrew K. Dunn, The University of Texas at Austin, Biomedical Engineering

Conference Dates

July 23-26, 2017

Abstract

The ability to visualize deep brain structures in vivo with high spatial resolution is of rising interest to investigate neuronal physiology and cerebral vasculature. Optical imaging offers non-invasive, high-resolution in vivo microscopy techniques to observe brain tissue and its surrounding environment. Two-photon fluorescence laser- scanning microscopy (2PM) can overcome depth limitations by using nonlinear excitation. The ideal approach for deep imaging in brain is to use both high energy pulses and longer excitation wavelengths.

Please click Additional Files below to see the full abstract.

Recommended Citation

David R. Miller, Ahmed Hassan, Jeremy W. Jarrett, Evan P. Perillo, Boris V. Zemelman, and Andrew K. Dunn, "In vivo multiphoton microscopy beyond 1 mm in the brain" in "Advances in Optics for Biotechnology, Medicine and Surgery XV", Peter So, Massachusetts Institute of Technology, USA Kate Bechtel, Triple Ring Technologies, USA Ivo Vellekoop, University of Twente, The Netherlands Michael Choma, Yale University, USA Eds, ECI Symposium Series, (2017). https://dc.engconfintl.org/biotech_med_xv/21