3D computational microscopy of dynamic samples
July 23-26, 2017
This talk will describe computational imaging methods for fast capture and reconstruction of 3D and 4D images in a commercial microscope. Our experimental setups employ inexpensive illumination-side and detection-side coding of angle (Fourier) space with simple hardware. The result is high-resolution intensity and phase images that span a large field-of-view, breaking the diffraction limit of the objective lens used and achieving high space- bandwidth-time product. We demonstrate real-time Gigapixel microscopy for in vitro biological cells and extend our methods to 3D imaging and algorithmic self-calibration. Through an end-to-end design of both the optical system and the computational algorithms, we achieve real-time 3D and phase recovery with digital aberration correction and mitigation of multiple scattering effects.
Laura Waller, "3D computational microscopy of dynamic samples" in "Advances in Optics for Biotechnology, Medicine and Surgery XV", Peter So, Massachusetts Institute of Technology, USA Kate Bechtel, Triple Ring Technologies, USA Ivo Vellekoop, University of Twente, The Netherlands Michael Choma, Yale University, USA Eds, ECI Symposium Series, (2017). https://dc.engconfintl.org/biotech_med_xv/6