Cortex-wide, cellular-resolution two-photon microscopy
June 2-6, 2019
Functional imaging of the mouse brain in its extreme complexity involves substantial trade-offs. An optical intrinsic spectroscopy system can image the entire cortex but at the expense of spatial and temporal resolution . A two-photon microscope (TPM) can image single neurons with high temporal resolution, but the field of view (FOV) is generally restricted. Advanced techniques like random-access scanning allow for imaging single neurons that are millimeters apart but only by ignoring the neurons and tissue in between . By carefully considering the properties of the optical components as well as the imaging requirements, we present a TPM capable of imaging nearly the entire mouse cortex with 15 Hz frame rates and single neuron resolution.
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Hunter B. Banks,; Jon R. Bumstead; Annie Bice; Joseph P. Culver; and Lindsey M. Brier, "Cortex-wide, cellular-resolution two-photon microscopy" in "Advances in Optics for Biotechnology, Medicine and Surgery XVI", Erin Buckley, Emory University/Georgia Institute of Technology, USA Christophe Moser, Polytechnique Fédérale de Lausanne (EPFL), Switzerland Brian Pogue, Dartmouth College, USA David Sampson, University of Western Australia, Australia Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/biotech_med_xvi/12