Neutron scattering: A versatile tool for probing complex solutions and surfaces
July 14-17, 2019
Neutron scattering has significant benefits for examining the structure of complex samples. Cold (slow) neutrons are non-damaging and predominantly interact with the atomic nucleus, meaning that they can penetrate deeply into samples, which allows for flexibility in the design of samples studied. Most importantly, there is a strong difference in neutron scattering length (i.e., scattering power) between protium ((_1^1)H, 99.98% natural abundance) and deuterium ((_1^2)H, or D, 0.015%). Through the mixing of H2O and D2O in the samples and in some cases the deuterium labelling of the biomolecules, components within a complex can be hidden or enhanced in the scattering signal. This enables both the overall structure and the relative distribution of components within a complex to be resolved. In this talk I will discuss the technical premise behind performing neutron scattering experiments and highlight examples where neutron scattering has been used to examine complex bio-pharmaceutical related phenomena. Examples will include in situ chromatography measurements and complex and realistic bio-mimetics at buried interfaces.
Luke Clifton, "Neutron scattering: A versatile tool for probing complex solutions and surfaces" in "Biological and Pharmaceutical Complex Fluids III: Protein Self-Assembly, Rheology and Interfacial Properties", Samiul Amin, Manhattan College, USA Miguel Rodrigues, University of Lisbon, Portugal Paolo Arosio, ETHZ, Switzerland Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/bpcf_iii/17