Arrest transitions in protein solutions – insight from combining scattering, microrheology, and computer simulations
July 14-17, 2019
The static and dynamic properties of concentrated protein solutions are essential ingredients for our understanding of the cellular machinery or formulating biopharmaceuticals. Here a combination of advanced characterization techniques such as light and x-ray scattering, neutron spin echo measurements  and microrheology experiments , combined with the theoretical toolbox from colloid physics and state-of-the-art computer simulations , considerably enhances our understanding of the link between protein interactions and the stability, dynamics and flow properties of these solutions up to high concentrations. We will address the enormous influence of weak attractive interactions known to exist between many globular proteins, and demonstrate the dramatic effect of an interaction potential anisotropy  such as attractive patches and shape anisotropy  on the dynamic properties. We will also discuss how we can combine interparticle interaction effects and the formation of (transient) equilibrium clusters in an attempt to understand and predict properties such as the concentration dependence of the zero shear viscosity of dense protein solutions .
(1) Bucciarelli, S.; Myung, J. S.; Farago, B.; Das, S., Vliegenthart, G.; Holderer, O.; Winkler, R. G.; Schurtenberger, P.; Gompper, G.; Stradner, A. “Dramatic influence of patchy attractions on short-time protein diffusion under crowded conditions” Sci. Adv. 2016, 2:e1601432.
(2) Garting, T. and Stradner, A. “Optical Microrheology of Protein Solutions using Tailored Nanoparticles” Small 2018, 1801548.
(3) Myung, J. S.; Roosen-Runge, F.; Winkler, R. G.; Gompper, G.; Schurtenberger, P.; Stradner, A. “Weak shape anisotropy leads to non-monotonic crowding effects impacting protein dynamics under physiologically relevant conditions” J. Phys. Chem. B 2018, 122, 12396-12402.
(4) Bergman, M.; Garting, T.; Schurtenberger, P.; Stradner, A. “Experimental Evidence for a Cluster Glass Transition in Concentrated Lysozyme Solutions” submitted to J. Phys. Chem. B, 2019.
Anna Stradner, "Arrest transitions in protein solutions – insight from combining scattering, microrheology, and computer simulations" in "Biological and Pharmaceutical Complex Fluids III: Protein Self-Assembly, Rheology and Interfacial Properties", Samiul Amin, Manhattan College, USA Miguel Rodrigues, University of Lisbon, Portugal Paolo Arosio, ETHZ, Switzerland Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/bpcf_iii/28