Protocol development to overcome bioprocess bottlenecks in the large-scale expansion of high quality hIPSC aggregates in vertical-wheel bioreactors
February 6 – 10, 2022
Human-induced pluripotent stem cells (hiPSCs) have generated a great deal of attention owing to their capacity for self-renewal and trilineage differentiation. hiPSCs are cultured as adherent colonies at small scale, which is sufficient to generate cells for experimental purposes but impractical to achieve large quantities for clinical applications. Bioreactor-based processes are the method of choice for efficient expansion and differentiation of cells. Current protocols for the expansion of hiPSCs, however, utilize horizontal impeller, paddle, or rocking wave mixing method bioreactors which require large static cell-culture starting populations and achieve only moderate cell fold increases within the bioreactor. We have recently demonstrated that the vertical-wheel bioreactor produces a unique fluid flow pattern that results in a homogeneous distribution of hydrodynamic forces, making it the opportune environment for systematic bioprocess optimization of hiPSC expansion.
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Breanna Borys, Tiffany Dang, Sunghoon Jung, Brian Lee, and Michael S. Kallos, "Protocol development to overcome bioprocess bottlenecks in the large-scale expansion of high quality hIPSC aggregates in vertical-wheel bioreactors" in "Advancing Manufacture of Cell and Gene Therapies VII", Sharon Brownlow, Cell & Gene Therapy Catapult, UK; Sean Palecek, University of Wisconsin, USA; Damian Marshall, Achilles Therapeutics, UK; Fernanda Masri, Cell & Gene Therapy Catapult, UK Eds, ECI Symposium Series, (2022). https://dc.engconfintl.org/cellgenetherapies_vii/17