Extra cellular vesicles separation and biophysical characterization
February 6 – 10, 2022
Cells bud off up to 10-fold of their biomass in form of extracellular vesicles. The term ‘extracellular vesicles’ refers to a heterogeneous population of vesicular bodies of cellular origin that derive either from the endosomal compartment (exosomes) or as a result of shedding from the plasma membrane (microvesicles, oncosomes and apoptotic bodies). Extracellular vesicles carry a variety of cargo, including RNA, proteins, lipids and DNA, which can be taken up by other cells, both in the direct vicinity of the source cell and at distant sites in the body via biofluids, and stimulate a variety of phenotypic responses. These functions of extracellular vesicles are not necessary when single cells are produced in a bioreactor for the plain purpose of biomass generation. The fact that cells in in-vitro culture release that many extracellular vesicles is completely neglected by research in the life science and biochemical engineering community. In addition, a lot of chromatin is present in cell culture supernatant. Due to the presence of chromatin the particle count is always overestimated due to the similar size between chromatin and extra cellular vesicles.
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Alois Jungbauer, Patricia Aguilar, Viktoria Mayer, and Alexander Zollner, "Extra cellular vesicles separation and biophysical characterization" in "Advancing Manufacture of Cell and Gene Therapies VII", Sharon Brownlow, Cell & Gene Therapy Catapult, UK; Sean Palecek, University of Wisconsin, USA; Damian Marshall, Achilles Therapeutics, UK; Fernanda Masri, Cell & Gene Therapy Catapult, UK Eds, ECI Symposium Series, (2022). https://dc.engconfintl.org/cellgenetherapies_vii/57