Discovering novel carbohydrate-active enzymes
September 24-28, 2017
Carbohydrate-active enzymes (CAZymes) mediate the assembly and breakdown of the multitude of complex carbohydrate structures that are found in nature. Because CAZymes find numerous applications in health and in industry, the discovery of novel enzymes is of wide interest. A sequence-based family classification of these enzymes was initiated over 25 years ago, and is continuously updated in the CAZy database (www.cazy.org). While the number of sequences of CAZymes continues to grow exponentially, the number of experimentally characterized enzymes grows at a much lower pace. Strikingly, the number of families keeps on growing at a steady rate, suggesting that many more CAZyme families are to be discovered. Traditionally CAZymes were identified (i) by serendipity, (ii) by screening against a particular substrate or (iii) by direct similarity to a previously characterized CAZyme. We will present various alternative methods that we have used recently to discover novel CAZymes and novel CAZyme families, including the module walk, the exploitation of genetic context and the systematic exploration of sequence space.
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Bernard Henrissat, "Discovering novel carbohydrate-active enzymes" in "Enzyme Engineering XXIV", Pierre Monsan, Toulouse White Biotechnology, France Magali Remaud-Simeon, LISBP-INSA, University of Toulouse, France Eds, ECI Symposium Series, (2017). https://dc.engconfintl.org/enzyme_xxiv/152