Enzymatic synthesis of cyclic imino acids
September 24-28, 2017
Optically active cyclic imino acids are widely used as important pharmaceutical intermediates and it is necessary to develop cost effective synthetic method for their production.
We have already established one-pot synthesis of L-cyclic imino acids from diamino acids by using N-methyl-L-amino acid dehydrogenase (NMAADH) from Pseudomonas putida. In order to make this process more efficient, we established a recombinant Escherichia coli which expresses NMAADH, lysine racemase from P. putida and D-lysine dehydrogenase from Selenomonas ruminantium in a single cell. The recombinant E. coli makes it possible to recycle NADPH by combination of reductive reaction using NMAADH and oxidative reaction using D-lysine dehydrogenase (Fig.1).
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Ryoma Miyake, Hiroshi Kawabata, Yasumasa Dekishima, and Takanobu Iura, "Enzymatic synthesis of cyclic imino acids" in "Enzyme Engineering XXIV", Pierre Monsan, Toulouse White Biotechnology, France Magali Remaud-Simeon, LISBP-INSA, University of Toulouse, France Eds, ECI Symposium Series, (2017). https://dc.engconfintl.org/enzyme_xxiv/31