Artificial (beta / alpha)8 barrel enzymes by in vitro evolution

Conference Dates

September 15-19, 2019


Natural evolution has yielded countless enzymes of the (β/α)8 barrel fold (TIM barrel). This versatile fold is highly favored by natural enzymes to catalyze a wide array of reactions and appears in five of the six enzymatic classes. Therefore, the (β/α)8 barrel fold is an attractive starting point for enzyme engineering. Several examples of modified TIM barrel enzymes have been reported that accept different substrates or even catalyze a different reaction. However, engineering new activities into the (β/α)8 barrel fold is apparently still far more challenging than its ubiquitous role in nature suggests.

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