Artificial (beta / alpha)8 barrel enzymes by in vitro evolution
September 15-19, 2019
Natural evolution has yielded countless enzymes of the (β/α)8 barrel fold (TIM barrel). This versatile fold is highly favored by natural enzymes to catalyze a wide array of reactions and appears in five of the six enzymatic classes. Therefore, the (β/α)8 barrel fold is an attractive starting point for enzyme engineering. Several examples of modified TIM barrel enzymes have been reported that accept different substrates or even catalyze a different reaction. However, engineering new activities into the (β/α)8 barrel fold is apparently still far more challenging than its ubiquitous role in nature suggests.
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Burckhard Seelig and Nisha Kanwar, "Artificial (beta / alpha)8 barrel enzymes by in vitro evolution" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/105