Artificial metalloenzymes for in vivo catalysis: Challenges and opportunities
September 15-19, 2019
Artificial metalloenzymes (ArMs) result from the incorportation of a catalyst precursor within a host protein, Figure 1. The resulting hybrid catalysts display features that are reminiscent of both homogeneous catalysts and enzymes. The optimization of the catalytic performance of ArMs is achieved by combining both chemical- and genetic means. The versatility of this chemo-genetic optimization strategy will be illustrated with selected examples including: transfer-hydrogenation, C–H activation, olefin metathesis, hydroamination etc, Figure 1.
With the aim of integrating artificial metalloenzymes in vivo, the second part of the talk will present our efforts to combine ArMs with natural enzymes to mimic essential features of the metabolism including: cascade reactions as well as up- and cross-regulation. Having identified the critical metabolites leading to ArM’s inhibition, our efforts towards engineering enzyme cascades in vivo will be summarized.
Please click Additional Files below to see the full abstract.
Thomas Ward, "Artificial metalloenzymes for in vivo catalysis: Challenges and opportunities" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/109