Substrate specificity of tRNA-dependent amide bond-forming enzyme
September 15-19, 2019
Streptothricins (STs) produced by Streptomyces strains are broad-spectrum antibiotics and are characterized by a streptothrisamine core structure with the L-β-lysine (β-Lys) residue and its oligomeric side chains [oligo(β-Lys)]. In addition to the STs, Streptomyces strains has been known to produce ST-related compounds, BD-12, citromycin, glycinothricin, etc., which possess a glycine-derived side chain rather than the β-Lys residue. We have reported that the amide bonds connecting the amino-acid side chains in ST and BD-12 are formed via NRPS (1) and tRNA-dependent (2) pathways, respectively (Figure 1).
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Yoshimitsu Hamano, "Substrate specificity of tRNA-dependent amide bond-forming enzyme" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/11