New engineered peptide ligases and substrate phage libraries for understanding cellular proteolysis
September 15-19, 2019
Proteolysis is one of nature’s major post-translational modifications and we need robust technologies to identify natural substrates and specificities for proteases. We have designed new peptide ligases capable of ligating peptide tags onto the N-terminus of newly proteolyzed proteins. These ligases allow identification of native protease substrates and cleavage sites both inside and outside of cells. We have also designed new substrate phage libraries coupled with deep sequencing to allow characterization of protease specificity. I’ll present these new technologies that we believe are general to characterizing protease clients and specificity.
James Wells, "New engineered peptide ligases and substrate phage libraries for understanding cellular proteolysis" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/124