Improved biomass conversion with Trichoderma reesei beta-glucosidase Cel3A engineered for broader substrate specificity
September 15-19, 2019
Fungal cellulolytic enzyme cocktails are widely used for the hydrolysis of biomass into fermentable sugars. Beta-Glucosidases enhance enzymatic biomass conversion by relieving cellobiose inhibition of endoglucanases and cellobiohydrolases. Fungal GH3 beta-glucosidases are often specific for hydrolysis of pyranose glycosides and severely inhibited by monomeric glucose.
Please click Additional Files below to see the full abstract.
Thijs Kaper, Amy Liu, Brad Kelemen, Zach Beck, Pim van der Kley, Slavko Kralj, Henrik Hansson, Mats Sandgren, and Nils Mikkelsen, "Improved biomass conversion with Trichoderma reesei beta-glucosidase Cel3A engineered for broader substrate specificity" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/23