Construction of thermostable enzymes

Conference Dates

September 15-19, 2019


The hyperthermophilic archaea were collected from the deep-sea in extreme environment, and the structures and functions of their hyperthermostable enzymes have been characterized. From our studies, the rules for the construction of thermostable enzymes have been discovered. Thermostable enzymes can be used for a number of industrial processes at higher temperature, taking advantage of reduced the risk of microbial contamination and solution viscosity as well as increase in the enzyme reaction rate. Using the information of sequence and structural homology among hyperthermostable enzymes, many types of thermostable enzymes have been constructed with protein engineering method.


[1] Crystal structure of beta-galactosidase from Bacillus circulans and the construction of the thermophilic mutants. FEBS Journal, vol 282,(13), pp2540-2552, 2015, K. Ishikawa, et al.

[2] Construction of thermophilic xylanase and Its structural analysis, Biochemistry, vol. 55, pp4399-4409, 2016, M. Watanabe, et al.

[3] Construction of thermostable cellobiohydrolase I from the fungus Talaromyces cellulolyticus by protein engineering, Protein Engineering, Design & Selection, vol. 1, pp1-8, 2019, M. Nakabayashi, et al.

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