Construction of thermostable enzymes
September 15-19, 2019
The hyperthermophilic archaea were collected from the deep-sea in extreme environment, and the structures and functions of their hyperthermostable enzymes have been characterized. From our studies, the rules for the construction of thermostable enzymes have been discovered. Thermostable enzymes can be used for a number of industrial processes at higher temperature, taking advantage of reduced the risk of microbial contamination and solution viscosity as well as increase in the enzyme reaction rate. Using the information of sequence and structural homology among hyperthermostable enzymes, many types of thermostable enzymes have been constructed with protein engineering method.
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 Construction of thermostable cellobiohydrolase I from the fungus Talaromyces cellulolyticus by protein engineering, Protein Engineering, Design & Selection, vol. 1, pp1-8, 2019, M. Nakabayashi, et al.
Kazuhiko Ishikawa, "Construction of thermostable enzymes" in "Enzyme Engineering XXV", Huimin Zhao, University of Illinois at Urbana-Champaign, USA John Wong, Pfizer, USA Eds, ECI Symposium Series, (2019). https://dc.engconfintl.org/enzyme_xxv/53